Novel Chip for Applying Mechanical Forces on Human Skin Models Under Dynamic Culture Conditions

In recent years the need for in vitro skin models as a replacement for animal studies has resulted in significant progress in the development of skin-on-a-chip models. These devices allow the fine control of the microenvironment of the model and the incorporation of chemical and physical stimuli. In this study, we describe the development of an easy and low-budget open-top dynamic microfluidic device for skin-on-a-chip experiments using polydimethylsiloxane and a porous polyethylene terephthalate membrane. The chip allows the incorporation of compressive stimuli during the cultivation period by the use of syringe pumps. Proof-of-concept results show the successful differentiation of the cells and establishment of the skin structure in the chip. The microfluidic skin-on-a-chip models presented in this study can serve as a platform for future drug and feasibility studies. Impact statement This microfluidic chip designed in this study allows easy application of dynamical stimuli. We show how to fabricate simple and cost-effective microfluidic chips and show the successful generation of reconstructed skin models. The presented data show skin models with a structure similar to native skin. Overall, the chip is an easy tool to study the effect of mechanical forces on skin equivalents.