The Morphometric Indicators of the Antibiotic Efficacy in E. coli Stimulated Human Blood Monocytes

Assessment of the different infections response to the antibiotics is a critical feedback process to ensure the proper coverage with antimicrobials. The need for rapid valuable test is mandatory for the clinicians to monitor their antibiotic treatment choice. The aim was to assess the digital imaging based morphometric analysis of peripheral blood monocyte cells (PBMC) response in relation to an antibiotic in an intention to be recommended as a routine rapid imaging-based antibiotic susceptibility testing. Peripheral blood monocytes were prepared by the technique of lymphoprep. The samples were divided into two parts; one was the test PBMCs-E. coli-ceftriaxone medium while the second is PBMCs-E. coli control medium. Both media were incubated and investigated by the standard dynamic image analysis using Mathwork R2021b software as image analyzer. There was a significant reduction in bacterial viability (CFU number), proliferation rate, movement activity and morphology in the efficiently treated E. coli sample. As well as, there was a significant enhancement of PBMCs phagocytic activity, movement rate and lysis for the human peripheral blood upon the proper use of ceftriaxone. The significant findings in the parameters used by this PBMCs-bacteria-antibiotic morphometric analysis test may confirm the feasibility and utility of the digital microscopic images montage for clinical monitoring of the efficiency of antibiotic coverage in patients with bacterial infections.