Establishment of acute hypergravity in vivo model

Introduction: Fighter and acrobatic flight pilots are exposed to short-term cerebral hypoperfusion or cerebral ischemia by hypergravity. Preventive therapy against hypergravity stress in pilots has been sought in recent years and needs to be developed in animal models as translational research. In this study, we developed acute hypergravity in vivo models, which can be gravity loaded arbitrarily, and the blood pressure, pulse rate, and hemodynamics over time during gravity loading can also be measured. Methods/Results: In this model, 9w male SD rats were anesthetized with sevoflurane (induction 5% for 4 min, maintenance 3%) by inserting catheters in the left femoral and right carotid arteries. After intraperitoneal injection including medetomidine 0.15 mg/kg + midazolam 2 mg/kg + butorphanol 2.5 mg/kg, the rats were loaded with+1Gz~ +5 Gz for 2 minutes by a hypergravity system (Tommy Seiko, Bio Research Center). We connected the system to the femoral catheter, and we used Labchart Software (ADInstruments) to monitor blood pressure and heart date during gravity loading. Then, we used i-stat(Abbot) to measure biomarkers, including lactate acid, via the right carotid catheter from 1min to 10min after gravity-loading Conclusion: Our acute hypergravity model enables us to monitor hemodynamics during gravity loading and measure the dynamics of biomarkers with short half-lives, such as lactic acid. This model applies to the evaluation of the kinetics of hypergravity-induced oxidative stress, including hydroxyl radicals and facilitate translational research. No Conflict of Interest This is the full abstract presented at the American Physiology Summit 2023 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.