X-ray phase contrast tomography with an immuno-histochemical approach

In the study of neurodegenerative diseases, a crucial point is the possibility of monitoring cells and tissues of interest within the body and tracking their modifications during the course of the disease or treatment. Currently, it is not possible to achieve this goal in-vivo, due to unsatisfactory image resolution, while conventional postmortem analysis necessitates the use of sample-destructive techniques. In this work, we propose to exploit the special characteristics of nanoparticles and nanobodies to enrich the abilities of nano and micro-XPCT, an innovative technique already used in the study of neurodegenerative disorders, and enhance it to tissue-selective 3D imaging, in addition to the morphological information provided by the 3D tomography.