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An Integrated Platform for the Assessment of Fluorescent Signals in Hydrogel Beads Produced by Droplet Microfluidics

Kathrine Nygaard Borg,Guangyao Cheng, Ayush Shetty, Tianle Wang,Cinzia Tesauro,Birgitta Ruth Knudsen,Yi-Ping Ho

2023 IEEE 23RD INTERNATIONAL CONFERENCE ON NANOTECHNOLOGY, NANO(2023)

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Abstract
Biomedical applications using hydrogel beads as incubators are continuously developing. Herein, we present a platform streamlining the production of hydrogel beads, detection of fluorescent signals, and post-analysis, using polyacrylamide (PAA) beads produced by droplet microfluidics as a model. A customized optical detection module is integrated with the data acquisition and analysis modules developed by LabVIEW and Python, respectively. The platform is evaluated by a series of characterizations. We expect to promote this platform for the quantitative assessment of biochemical reactions occurring in the PAA-based incubators.
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Key words
analysis modules,biochemical reactions,biomedical applications,customized optical detection module,data acquisition,droplet microfluidics,fluorescent signals,hydrogel beads,LabVIEW,PAA-based incubators,polyacrylamide beads,Python,quantitative assessment
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