Expression profiles of human somatic mesenchymal stem cells derived from fresh endometrium, ectopic-endometrium and umbilical cord
GINEKOLOGIA POLSKA(2023)
摘要
Objectives:The study investigated the stem cell expression profiles and differentiation capacities of mesenchymal stem cells (MSCs) from different tissues, specifically human eutopic endometrium MSCs (eut-MSCs), ectopic endometrium MSCs (ect-MSCs), and umbilical cord MSCs (UC-MSCs). Our aim was to identify any similarities in subpopulations among these MSCs and lay a foundation for MSCs repair.Material and methods: MSCs were isolated from endometrial tissue (n = 5), endometriosis tissue (n = 6), and umbilical cords (n = 7). Flow cytometry was used to examine cell phenotype, and three lineage tests were conducted to evaluate the differentiation capacity of the MSCs.Results: Eut-MSCs expressed CD44 (98.00 +/- 0.96%), CD73 (99.54 +/- 0.02%), CD140b (99.16 +/- 0.50%), CD146 (93.87 +/- 2.27%), SUSD2 (50.76 +/- 8.15%), and CD271 (2.1 +/- 1.22%). Ect-MSCs expressed CD44 (98.23 +/- 1.60%), CD73 (99.63 +/- 0.04%), CD140b (98.13 +/- 0.53%), CD146 (93.88 +/- 3.19%), SUSD2 (49.33 +/- 6.36%), and CD271 (2.85 +/- 1.17%). UC-MSCs expressed CD44 (99.11 +/- +/- 0.42%), CD73 (99.65 +/- 0.12%), CD140b (99.84 +/- 0.42%), CD146 (88.09 +/- 4.20%), SUSD2 (72.87 +/- 7.13%), and CD271 (6.19 +/- +/- 2.08%). The expression of SUSD2 and CD271 in UC-MSCs was slightly but not significantly higher than that in ect-MSCs and eut-MSCs. However, CD44, CD73, CD140b, and CD146 showed similar expression levels in UC-MSCs, ect-MSCs, and eutMSCs. All three types of MSCs demonstrated the capacity to differentiate into osteoblasts, adipocytes, and chondrocytes.Conclusions: Our findings indicate that ect-MSCs, eut-MSCs, and UC-MSCs have similar stem cell phenotypes and the ability to differentiate into three lineages.
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关键词
endometrium,umbilical cord,mesenchymal stem cells,phenotypic expression,differentiation potential
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