Growth condition for over production of odd-chain fatty acids in the methylotrophic yeast Komagataella phaffii GS115

C17 fatty acids, which are odd-chain fatty acids (OcFAs), have attractive commercial and medical value. Komagataella phaffii, a promising species of methylotrophic yeast, has received increasing attention due to its unique features. Especially, the insanely higher yield of C17 fatty acids in fer-mentation, compared with other methylotrophic yeast species, is expected to confirm K. phaffii as a promising biorefinery host for production of OcFAs. Moreover, co-supplementation of 0.1% propionate and 10 mM valine in a glucose-based medium, increased the productivity of C17 fatty acids in K. phaffii by 11.7-fold, and its production reached 4.36 mg/L. This research presents new insights into a non-genetically engineered method of OcFAs biosynthesis in K. phaffii.