Sex Genotyping Mice by Polymerase Chain Reaction.

Virginia E Papaioannou,Richard R Behringer

Cold Spring Harbor protocols（2024）

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摘要

A simple method to determine the genetic sex of a mouse is to amplify DNA from a male-specific gene by polymerase chain reaction (PCR). This protocol is used to detect the Y-chromosome-specific gene Sry in tissue lysates of tail tip or ear punch samples.

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Gene Expression

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