Biodegradation and sorption of nutrients and endocrine disruptors in a novel concrete-based substrate in vertical-flow constructed wetlands.

Removing phosphorus and endocrine-disruptors (EDC) is still challenging for low-cost sewage treatment systems. This study investigated the efficiency of three vertical-flow constructed wetlands (VFCW) vegetated with Eichhornia crassipes onto red clay (CW-RC), autoclaved aerated concrete (CW-AC), and composite from the chemical activation of autoclaved aerated concrete with white cement (CW-AAC) in the removal of organic matter, nutrients, and estrone, 17β-estradiol, and 17α-ethinylestradiol. The novelty aspect of this study is related to selecting these clay and cementitious-based materials in removing endocrine disruptors and nutrients in VFCW. The subsurface VFCW were operated in sequencing-batch mode (cycles of 48-48-72 h), treating synthetic wastewater for 308 days. The operation consisted of Stages I and II, different by adding EDC in Stage II. The presence of EDC increased the competition for dissolved oxygen (OD) and reduced the active sites available for adsorption, diminishing the removal efficiencies of TKN and TAN and total phosphorus in the systems. CW-RC showed a significant increase in COD removal from 65% to 91%, while CW-AC and CW-AAC maintained stable COD removal (84%-82% and 78%-81%, respectively). Overall, the substrates proved effective in removing EDC, with CW-AC and CW-AAC achieving >60% of removal. Bacteria Candidatus Brocadia and Candidatus Jettenia, responsible for carrying out the Anammox process, were identified in assessing the microbial community structure. According to the mass balance analysis, adsorption is the main mechanism for removing TP in CW-AC and CW-AAC, while other losses were predominant in CW-RC. Conversely, for TN removal, the adsorption is more representative in CW-RC, and the different metabolic routes of microorganisms, biofilm assimilation, and partial ammonia volatilization in CW-AC and CW-AAC. The results suggest that the composite AAC is the most suitable material for enhancing the simultaneous removal of organic matter, nutrients, and EDC in VFCW under the evaluated operational conditions.