Anthropogenic Transmission of SARS-CoV-2 from Humans to Lions, Singapore, 2021

In Singapore, 10 captive lions tested positive for SARS-CoV-2 by real-time PCR. Genomic analyses of nanopore sequencing confirmed human-to-animal transmission of the SARS-CoV-2 Delta variant. Viral genomes from the lions and zookeeper shared a unique spike protein substitution, S:A1016V. Widespread SARS-CoV-2 trans-mission among humans can increase the likelihood of anthroponosis.