Comprehensive Analysis of Phenolic Compounds, Carotenoids, and Antioxidant Activities in Lactuca sativa var. longifolia Cultivated in a Smart Farm System

Smart farming is a promising strategy for future agriculture, and the global market for smart agriculture is growing rapidly. The aim of this study was to compare the quality of agricultural products based on their cultivation method. The components and antioxidant activities of Lactuca sativa (LS) var. longifolia cultivated using conventional agriculture methods (CAMs) and smart farm agriculture methods (SAMs) were compared. ABTS+ and DPPH radical scavenging activity assays were performed to compare the antioxidant activities of LS grown using CAMs and SAMs. High-performance liquid chromatography with a reverse-phase column was employed to analyze the phenolic and carotenoid compounds. In both the SAM and the CAM, beta-carotene was found to be the most abundant compound. LS grown using the CAM had high total polyphenol content and ABTS+ radical scavenging activity, while LS grown using the SAM had high individual phenolic content and DPPH radical scavenging activity. This study developed a new method of SAM and confirmed that SAM produces a higher quantity of phenolic compounds and total flavonoid contents than CAM in LS. This study also highlights the SAM's functioning, gives crop quality inspection guidelines, and standardizes LS. The new SAM is effective for application in undeveloped urban spaces, is more convenient than the CAM, and represents a way to develop and manage LS quality.