A framework for assessing variations in ecological networks to support wildlife conservation and management

•The first comprehensive framework for examining variations in ecological networks.•The scalability of the framework makes it valuable for explore the impact of environment on ecological networks under diverse scenarios.•We introduced a novel method considering the importance and human disturbance faced by core habitats to identify priority areas for protection.•We stressed the significance of considering changes of ecological network and human disturbance on core habitats to promote wildlife conservation and human-wildlife coexistence.