Introgression of 1RS WR .1BL translocation, Bx7 OE and stripe rust resistance genes in bread wheat cultivars

Sheenu Garg,Ambika Sharma,Satinder Kaur, H. S. Dhaliwal, Pritesh Vyas,Imran Sheikh

Cereal Research Communications(2023)

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摘要
The composition of seed storage proteins determines the suitability of bread wheat cultivar for different end-use products. high-molecular-weight glutenin subunits are the major determinants of the processing quality of bread wheat flour. The spontaneous translocation of the 1RS arm of Secale cereale L. in bread wheat is associated with higher grain yield and abiotic stress tolerance, but it leads to deterioration in the end-use quality due to the presence of rye storage secalin protein encoded by Sec-1 locus. The 1RS WR .1BL recombinant line W1635 without Sec-1, over-expressed Glu1- Bx7 ( Bx7 OE ) and two stripe rust resistance genes Yr5 and Yr17 were used as the donor for marker-assisted breeding in the bread wheat cultivars UP2338 and HS240 with 1RS.1BL translocation. A strong positive correlation was found between the micro-SDS sedimentation test (MST) values and Bx7 OE in the derivatives along with Glu-D1 (5 + 10) indicating the efficacy of Bx7 OE in improving the end-use quality. In addition to this, the introgression of Yr5 and Yr17 genes led to stripe rust resistance in the derivatives for their stable and environment friendly production.
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关键词
1RS.1BL translocation,Yr5,Yr17,High-molecular-weight glutenin subunit,Glu1-Bx7 over-expression,Micro-SDS Sedimentation Test
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