Daptomycin production enhancement by ARTP mutagenesis and fermentation optimization in Streptomyces roseosporus.

This study successfully screened a high yield strain of daptomycin through a double-reporter system combined with ARTP mutation. The expression level of two-reporter genes can evaluate the strength of dptEp promoter, which can stimulate the expression level of dptE in the biosynthesis of daptomycin, thus producing more daptomycin. The developed multi-stage feeding rate strategy provides a novel way to increase daptomycin in industrial fermentation.