Logarithmic total variation regularization via preconditioned conjugate gradient method for sparse reconstruction of bioluminescence tomography

Background and objective: Bioluminescence Tomography (BLT) is a powerful optical molecular imaging technique that enables the noninvasive investigation of dynamic biological phenomena. It aims to reconstruct the threedimensional spatial distribution of bioluminescent sources from optical measurements collected on the surface of the imaged object. However, BLT reconstruction is a challenging ill-posed problem due to the scattering effect of light and the limitations in detecting surface photons, which makes it difficult for existing methods to achieve satisfactory reconstruction results. In this study, we propose a novel method for sparse reconstruction of BLT based on a preconditioned conjugate gradient with logarithmic total variation regularization (PCG-logTV). Method: This PCG-logTV method incorporates the sparsity of overlapping groups and enhances the sparse structure of these groups using logarithmic functions, which can preserve edge features and achieve more stable reconstruction results in BLT. To accelerate the convergence of the algorithm solution, we use the preconditioned conjugate gradient iteration method on the objective function and obtain the reconstruction results. We demonstrate the performance of our proposed method through numerical simulations and in vivo experiment. Results and conclusions: The results show that the PCG-logTV method obtains the most accurate reconstruction results, and the minimum position error (LE) is 0.254mm, which is 26%, 31% and 34% of the FISTA (0.961), IVTCG (0.81) and L1-TV (0.739) methods, and the root mean square error (RMSE) and relative intensity error (RIE) are the smallest, indicating that it is closest to the real light source. In addition, compared with the other three methods, the PCG-logTV method also has the highest DICE similarity coefficient, which is 0.928, which means that this method can effectively reconstruct the three-dimensional spatial distribution of bioluminescent light sources, has higher resolution and robustness, and is beneficial to the preclinical and clinical studies of BLT.