A Highly Selective Ratiometric Fluorescent Probe for the Detection of Hypochlorite in Acute Lung Injury

The fluorescent probe for the detection of overexpressed ClO- in acute lung injury is required to have high selectivity against other reactive species. A hydroxybenzo[g]coumarin, NaphCou, was synthesized as a ratiometric fluorescent probe to detect ClO- with ultra-high selectivity, a fast response, long-wavelength emissions (from green to red) and large Stokes shifts (from 107 nm to 155 nm). Using probe NaphCou as a fluorescence sensing tool, the lipopolysaccharide (LPS)-induced acute lung injury in mouse model was investigated by monitoring the hypochlorous acid concentration variation.