Comparative effect of platelet- and mesenchymal stromal cell- derived extracellular vesicles on human cartilage explants using an ex vivo inflammatory osteoarthritis model

Bone & joint research(2023)

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摘要
AimsExtracellular vesicles (EVs) are nanoparticles secreted by all cells, enriched in proteins, lipids, and nucleic acids related to cell- to- cell communication and vital components of cell- based therapies. Mesenchymal stromal cell (MSC)-derived EVs have been studied as an alternative for osteoarthritis (OA) treatment. However, their clinical translation is hindered by industrial and regulatory challenges. In contrast, platelet-derived EVs might reach clinics faster since platelet concentrates, such as platelet lysates (PL), are already used in therapeutics. Hence, we aimed to test the therapeutic potential of PL-derived extracellular vesicles (pEVs) as a new treatment for OA, which is a degenerative joint disease of articular cartilage and does not have any curative or regenerative treatment, by comparing its effects to those of human um-bilical cord MSC-derived EVs (cEVs) on an ex vivo OA-induced model using human cartilage explants.MethodspEVs and cEVs were isolated by size exclusion chromatography (SEC) and physically charac-terized by nanoparticle tracking analysis (NTA), protein content, and purity. OA conditions were induced in human cartilage explants (10 ng/ml oncostatin M and 2 ng/ml tumour necrosis factor alpha (TNF alpha)) and treated with 1 x 109 particles of pEVs or cEVs for 14 days. Then, DNA, glycosaminoglycans (GAG), and collagen content were quantified, and a histo-logical study was performed. EV uptake was monitored using PKH26 labelled EVs.ResultsSignificantly higher content of DNA and collagen was observed for the pEV-treated group compared to control and cEV groups. No differences were found in GAG quantification nor in EVs uptake within any treated group.ConclusionIn conclusion, pEVs showed better performance than cEVs in our in vitro OA model. Although further studies are needed, pEVs are shown as a potential alternative to cEVs for cell- free re-generative medicine.
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关键词
Platelet lysate,Osteoarthritis,Extracellularvesicles,Regenerative medicine,Cartilage repair,Human umbilical cord mesenchymal stromal cells
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