m7GHub V2.0: an updated database for decoding the N7-methylguanosine (m⁷G) epitranscriptome

With recent progress in mapping N7-methylguanosine (m(7)G) RNA methylation sites, tens of thousands of experimentally validated m(7)G sites have been discovered in various species, shedding light on the significant role of m(7)G modification in regulating numerous biological processes including disease pathogenesis. An integrated resource that enables the sharing, annotation and customized analysis of m(7)G data will greatly facilitate m(7)G studies under various physiological contexts. We previously developed the m7GHub database to host mRNA m(7)G sites identified in the human transcriptome. Here, we present m7GHub v.2.0, an updated resource for a comprehensive collection of m(7)G modifications in various types of RNA across multiple species: an m7GDB database containing 430 898 putative m(7)G sites identified in 23 species, collected from both widely applied next-generation sequencing (NGS) and the emerging Oxford Nanopore direct RNA sequencing (ONT) techniques; an m7GDiseaseDB hosting 156 206 m(7)G-associated variants (involving addition or removal of an m(7)G site), including 3238 disease-relevant m(7)G-SNPs that may function through epitranscriptome disturbance; and two enhanced analysis modules to perform interactive analyses on the collections of m(7)G sites (m7GFinder) and functional variants (m7GSNPer). We expect that m7Ghub v.2.0 should serve as a valuable centralized resource for studying m(7)G modification. It is freely accessible at: www.rnamd.org/m7GHub2.