Baseline micronucleus frequencies and ⁶⁰Co cytokinesis-block micronucleus assay dose-response curve for biodosemetry in Vietnam

This study aims to establish baseline micronucleus (MN) frequencies from various populations of residents in Vietnam and develop a Co-60 dose-response curve for the cytokinesis-block micronucleus (CBMN) assay. Blood samples were exposed in vitro to a Co-60 source at a dose rate of 275 mGy per min in a range of 0.1 to 4.0 Gy. MN background frequencies were 4.5 +/- 3.2, 7.3 +/- 4.6, 7.0 +/- 3.8 and 13.1 +/- 6.7 in 1000 binucleated (BN) cells for 96 healthy donors, 22 male radiation workers and 12 breast cancer patients, respectively. Blood samples from three healthy donors were used to generate the MN dose-response curve: y = C + (0.0496 +/- 0.0069)D + (0.0143 +/- 0.0026)D-2. This curve was verified through an inter-laboratory comparison (RENEB ILC 2021). Our findings highlight the significance of the CBMN assay as an additional essential tool for biodosemetry in Vietnam.