Phosphorylation modification orchestrates the functionalities of peroxin 14 in filamentous entomopathogenic fungus Beauveria bassiana

Peroxin 14 (Pex14) is a component of the receptor-docking complex at peroxisomal membrane. However, its post translation modification remains largely unknown in filamentous fungi. In this study, we char-acterized two phosphorylation sites (S54 and T262) in Beauveria bassiana Pex14 (BbPex14). Two phos-phorylation sites are dispensable for the BbPex14 role as a peroxin. The BbPex14 roles in conidiation and blastospore formation are dependent on two phosphorylation sites, and blastospore formation is more dependent on phosphorylation modification of two sites. Two phosphorylation sites differentially contribute to pexophagy during conidiation and under stress, in which the site T262 is indispensable. Evidently, the phosphorylation modification expands the functionalities of BbPex14. This study improves our understandings of the complex regulatory mechanisms underlying organellar biology in the fila-mentous fungi.& COPY; 2023 British Mycological Society. Published by Elsevier Ltd. All rights reserved.