Sporolactobacillus mangiferae sp. nov., a spore- forming lactic acid bacterium isolated from tree bark in Thailand

A Gram- stain-positive, facultatively anaerobic and endospore-forming rod-shaped bacterium, designed strain CPB3- 1T, was isolated from tree bark. This homofermentative strain produced D & iota;-lactic acid from glucose. It grew at 20-45 & DEG;C, pH 4.0-9.5 and in 0-3.0 % (w/v) NaCl. It contained meso- diaminopimelic acid in cell -wall peptidoglycan and had menaquinone with seven isoprene units (MK -7) as the predominant component. The major fatty acid was anteiso-C17:0. The polar lipids were phosphatidylglycerol, diphosphatidylglycerol, an unknown phospholipid and an unknown lipid. Based on the results of 16S rRNA gene sequence analysis, strain CPB3- 1T belonged to the genus Sporolactobacillus and was closely related to Sporolactobacillus kofuensis DSM 11701T and Sporolactobacillus spathodeae BK117-1T (both 96.7 % similarity), Sporolactobacillus inulinus NRIC 1133T and Sporolactobacillus terrae DSM 11697T (both 96.6 % similarity), and Sporolactobacillus shoreicorticis MK21-7T, Sporolactobacillus laevolacticus DSM 442T, Sporolactobacillus shoreae BK92T and Sporolactobacillus pectinivorans GD201205T (all 95.8-96.5 % similarity). The draft genome of strain CPB3- 1T contained 2 930 919 bps with 3117 coding genes. The DNA G+C content was 45.1 mol%. The digital DNA-DNA hybridization values between strain CPB3- 1T and closely related type strains were 19.2-24.0 %. The average nucleotide identity (84.0-87.6 %) and average amino acid identity (66.5-76.3 %) values were lower than the cut -off values for species delineation. Strain CPB3- 1T was clearly distinguished from related Sporolactobacillus species based on its phenotypic and chemotaxonomic characteristics, 16S rRNA gene sequence similarity and the results of draft genome analysis. Therefore, the strain represents a novel species of the genus Sporolactobacillus, for which the name Sporolactobacillus mangiferae sp. nov. is proposed. The type strain is CPB3- 1T (=JCM 35082T=TISTR 10004T).