Physiological factors contribute to increased competitiveness of grass relative to sedge, forb and legume species under different N application levels

In alpine grasslands, increased N deposition is increasing the dominance of grasses relative to other functional types according to our previous study Shen et al. (2022). However, the mechanisms that drive this compositional change are not fully understood. We measured the effects of 4-6 years' N addition to simulate N deposition at rates of 0 (CK), 8 (N1), 24 (N2), 40 (N3), 56 (N4), and 72 (N5) kg N ha-1 year-1 on dominant representatives of four functional types, Leymus secalinus (grass), Carex capillifolia (sedge), Potentilla multidi (non-leguminous forb), and Medicago ruthenica (legume), in the alpine grassland on the Qinghai -Tibetan Plateau (QTP). In -situ experiment showed that N addition increased aboveground biomass in L. secalinus but had negative or neutral effects on aboveground biomass in the other species. Consistent with this finding, N addition increased net photosynthesis, chlorophyll content, and rubisco activity in L. secalinus with less positive effects on the other species. Nitrogen addition increased leaf N content in L. secalinus and C. capillifolia and reduced leaf nonstructural carbohydrate content in all four species. In L. secalinus, the highest N addition rate (N5) reduced MDA content, a marker of oxidative stress, by enhancing antioxidant enzyme activity. Overall, our findings suggested that physiological factors can contribute to increased competitiveness of grass relative to sedge, forb and legume species under high N application levels. The rapid growth of this grass species reduces resource availability to non -grass species, increasing its dominance in the alpine meadow.