Leveraging existing 16S rRNA microbial data to identify diagnostic biomarker in Chinese patients with gastric cancer: a systematic meta-analysis

Gastric cancer is the second most prevalent and deadly cancer in China. Microbiota play an important role in gastric tumorigenesis. However, the available microbial marker studies for gastric cancer do not have consistent results. We searched PubMed for 16S rRNA sequencing in relevant literature on Chinese patients from 1 January 2005 to 18 July 2022, and 16 original articles were finally obtained. Alpha diversity, beta diversity, and bacterial taxa were used to explore the differences in gastric microbiota. Linear discriminant analysis of effect size and a random forest model were used to find the combination of genera with the best diagnostic efficacy. Streptococcus, Pseudomonas, Fusobacterium, Selenomonas, Peptostreptococcus, and Prevotella showed significant differences between gastric cancer and non-gastric cancer, but a single genus performed poorly in identifying patients with gastric cancer. However, a combination of genera Streptococcus, Peptostreptococcus, Selenomonas, Pseudomonas, and Prevotella had excellent performance in screening gastric cancer with the median area under the curve values of 0.7525 (range: 0.5859-0.9350), 0.8818 (range: 0.7397-0.9533), and 0.7435 (range: 0.7131-0.8483) in the Matched, Unmatched, and Other groups, respectively. Therefore, the results indicated that this combination of genera has good diagnostic efficacy and wide applicability for patients with gastric cancer, which may provide new clues for the non-invasive diagnosis of gastric cancer.