Analyzing the atypical – Methods for studying the menstrual cycle in adolescents

•Ovulation determination methods for adults cannot be applied to adolescents.•Relational methods to determine LH peak & PdG rise are essential for ovulation detection.•Daily hormone measurements are recommended, LH testing needs to be modified to sample.•Counting methods need to be used with caution in samples with high rates of anovulation.•Recommendations for menstrual cycle research in peripuberty are discussed.