Associations of reproductive and breastfeeding history with anti-Mullerian hormone concentration among African- American women of reproductive age

Research question: Are gravidity, parity and breastfeeding history associated with anti-Mueurollerian hormone concentration among African-American women of reproductive age? Design: This study included baseline data from the Study of the Environment, Lifestyle and Fibroids, a 5-year longitudinal study of African-American women. Within this community cohort, data from 1392 women aged 25-35 years were analysed. The primary outcome was serum anti-Mueurollerian hormone concentration measured using the Ansh Labs picoAMH assay, an enzyme-linked immunosorbent assay. Multivariable linear regression models were used to estimate mean differences in anti-Mueurollerian hormone concentration (b) and 95% CI by self-reported gravidity, parity and breastfeeding history, with adjustment for potential confounders. Results: Of the 1392 participants, 1063 had a history of gravidity (76.4%). Of these, 891 (83.8%) were parous and 564 had breastfed. Multivariable-adjusted regression analyses found no appreciable difference in anti-Mueurollerian hormone concentration between nulligravid participants and those with a history of gravidity (b =-0.025, 95% CI-0.145 to 0.094). Among participants with a history of gravidity, there was little difference in anti-Mueurollerian hormone concentration between parous and nulliparous participants (b = 0.085, 95% CI-0.062 to 0.232). There was also little association between anti-Mueurollerian hormone concentration and breastfeeding history (ever versus never: b = 0.009, 95% CI-0.093 to 0.111) or duration of breastfeeding (per 1-month increase: b =-0.002, 95% CI-0.010 to 0.006). Conclusions: Gravidity, parity and breastfeeding history were not meaningfully associated with anti-Mueurollerian hormone concentration in this large sample of the Study of the Environment, Lifestyle and Fibroids cohort.