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Short-term assays for mesenchymal stromal cell immunosuppression of T-lymphocytes.

Frontiers in immunology(2023)

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Abstract
Takeaways from these studies are as follows: (1) while early measures of PBMC activation is evident at 2-6 h, immunosuppression was only reliably detected at 24 h; (2) PS externalization at 24 h is a surrogate assay for MSC immunomodulation; and (3) rapid ELISA assay detection of TNFα release by PBMCs is a robust and sensitive assay for MSC immunomodulation at 24 h.
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Key words
IL-6,caspase,cytokine,mesenchymal stromal cell,peripheral blood mononuclear cells,phosphatidyl serine,tNF-alpha
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