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A protocol for whole-brain Ca2+ imaging in Astyanax mexicanus, a model of comparative evolution

Evan Lloyd,Martin Privat,German Sumbre,Erik R. Duboue,Alex C. Keene

STAR PROTOCOLS（2023）

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Abstract
In this protocol, we describe a comparative approach to study the evolution of brain function in the Mexican tetra, Astyanax mexicanus. We developed surface fish and two independent populations of cavefish with pan-neuronal expression of the Ca2+ sensor GCaMP6s. We describe a methodology to prepare samples and image activity across the optic tectum and olfactory bulb.
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Neuroscience,Evolutionary Biology
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