Matrices-charges of agar-alginate crosslinked microcapsules via o/w microemulsion: A non-spore forming probiotic bacteria encapsulation system for extensive viability.

Non-spore forming probiotic bacteria tend to diminish their activity under adverse conditions. This leads to the difficulty of delivery in animal body system as well as the feed pelleting process. The present work proposes the microcapsule networks based on polymer matrices and charges under ionic crosslink to encapsulate probiotic for an extensive stability in adverse conditions. The combination of agar (AG) and alginate (AL) is a good model to combine agar matrices and alginate charges under ionic crosslink through o/w emulsion system for probiotic incorporation. By simply mixing Lactobacillus plantarum MB001(LPMB001) with AL and AG containing few drops of soybean oil, a stable o/w microemulsion can be obtained. The addition of calcium chloride favors the ionic crosslink among AG matrices resulting in LPMB001/AG-AL microcapsules. In vitro studies indicate the survival of LPMB001 and the slow release even after treatment in adverse conditions. This microencapsulation prolongs LPMB001 viability under the heat treatment and the storage conditions and this designates the potential feed processing. The present work for the first time shows how we can combine polymer matrices and charges to protect probiotic from the adverse conditions which is simple and practical for the process of dietary supplementation.