High-throughput creation of Nicotiana tabacum gene-targeted mutants based on CRISPR/Cas9
Plant cell reports（2023）
Abstract
Key message 4382 available sgRNAs targeting 1060 tobacco genes were obtained, and 10,682 targeted mutants were created using high-throughput methods. Four optimization experiments were established to solve problems encountered during genetic transformation.
MoreTranslated text
Key words
crispr/cas9,crispr/cas9,mutants,high-throughput,gene-targeted
AI Read Science
Must-Reading Tree
Example
Generate MRT to find the research sequence of this paper
Chat Paper
Summary is being generated by the instructions you defined