Ultrafast Subcellular Biolabeling and Bioresponsive Real-Time Monitoring for Targeting Cancer Theranostics

Cell heterogeneity poses a formidable challenge for tumor theranostics, requiring high-resolution strategies for intercellular bioanalysis between single cells. Nanoelectrode-based electrochemical analysis has attracted much attention due to its advantages of label-free characteristics, relatively low cost, and ultra-high resolution for single-cell analysis. Here, we have designed and developed a subcellular biolabeling and bioresponsive real-time monitoring strategy for precise bioimaging-guided cancer diagnosis and theranostics. Our observations revealed the apparent intracellular migration of biosynthetic Au nanoclusters (Au NCs) at different subcellular locations, i.e., from the mitochondria to the mitochondrion-free region in the cytoplasm, which may be helpful for controlling over the biosynthesis of Au NCs. Considering the precise biolabeling advantage of the intracellular biosynthetic Au NCs for biomedical imaging of cancers, it is important to realize the biosynthetic Au NC-enabled precise control in real-time theranostics of cancer cells. Therefore, this work raises the possibility to achieve subcellular monitoring of H2O2 for targeting cancer theranostics, thereby providing a new way to explore the underlying mechanism and imaging-guided tumor theranostics.