Phytochemical constituents from rhizomes of Dryopteris crassirhizoma and their anti-inflammatory activity.

The phytochemical investigation on the rhizomes of (Dryopteridaceae) resulted in the discovery of one novel compound, drycrassirhizomamide A (), and one new natural product, drycrassirhizomamide B (), as well as four known isolates, ()-(-)--benzoylphenylalaninol (), blumenol A (), 8--glucosylnoreugenin (), and dryopteroside (). Their chemical structures were identified by NMR and mass spectroscopy. Compounds were determined to be 1,19-diethyl 10-oxo-2,9,11,18-tetraazanonadecanedioate and ,'-diethyl ,'-1,6-hexanediylbis[carbamate]. The anti-inflammatory activities of these compounds were evaluated with LPS-stimulated RAW264.7 macrophage and BV2 microglia. The results showed that compounds and have inhibitory effects of NO production with IC values of 13.41, 30.36, 25.51, and 11.35 μM in LPS-stimulated RAW264.7 cells. Also, compounds and have abilities to inhibit NO production with the IC values of 40.11, 30.94, 15.76, and 16.79 μM in BV2 cells, which demonstrated that they may possess the potential anti-inflammatory activity.