Genome-Wide RNA Tomography in the Mouse Whole Olfactory Mucosa.
Methods in molecular biology (Clifton, N.J.)（2023）
Abstract
Spatial transcriptomics allows for the genome-wide profiling of topographic gene expression patterns within a tissue of interest. Here, we describe our methodology to generate high-quality RNA-seq libraries from cryosections from fresh frozen mouse whole olfactory mucosae. This methodology can be extended to virtually any vertebrate organ or tissue sample.
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Key words
tomography,genome-wide
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