Secretory Production of 7-dehydrocholesterol by Engineered Saccharomyces cerevisiae.
Biotechnology journal（2023）
Abstract
Selective secretion of different sterol intermediates was characterized in sterol over-produced strain and the extracellular export of 7-DHC developed in present study significantly improved the cell biosynthetic capacity, which offered a novel modification idea for 7-DHC de novo biosynthesis by S. cerevisiae cell factory. This article is protected by copyright. All rights reserved.
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Key words
biphasic fermentation, 7-dehydrocholesterol, lipid transporter protein, metabolic engineering, molecular modification, sterol transporter
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