Novel dual-recognition electrochemical biosensor for the sensitive detection of AFM₁ in milk

Aflatoxin M1 (AFM1) is a highly carcinogenic and toxic compound that is commonly found in dairy foods such as milk. We developed an electrochemical MIP (molecularly imprinted polymer) and aptamer (Apt) based aptasensor (Apt-Au@PEIM/AFM1/MIP-Apt/AuNPs)/GCE) with dual-recognition signal amplification to detect AFM1 in milk. The MIP-Apt/AuNP/GCE sensor was prepared by synthesizing an MIP film through the electropolymerization of resorcinol on gold nanoparticles-modified electrodes. The dropwise addition of our cAptAu@PEIM signal amplification probe could enhance the current signal response and improve the sensitivity of the sensor to detect AFM1. The AFM1 present in milk could prevent the probe from binding to the sensor, thereby drastically reducing the current signal. Under optimal experimental conditions, the sensor exhibits a linear range of 0.01-200 nM and a limit of detection of 0.07 nM (S/N = 3). The developed dual-recognition aptasensor would provide a novel and rapid method for specific detection of AFM1 in milk.