qMaLioffG: A single green fluorescent protein FLIM indicator enabling quantitative imaging of endogenous ATP
bioRxiv (Cold Spring Harbor Laboratory)(2023)
摘要
The widespread use of fluorescence lifetime imaging microscopy (FLIM) for quantitative imaging is hindered by the limited availability of a FLIM-based genetically encoded indicator using a conventional 488 nm laser. Here, we present qMaLioffG, a single green fluorescent protein FLIM indicator showing a fluorescence lifetime change in ATP concentration within the physiological range. This allows quantitative imaging of endogenous ATP to investigate cellular energy status of different cell types.
### Competing Interest Statement
The authors have declared no competing interest.
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关键词
atp,quantitative imaging,protein
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