Introgression of recombinant 1RS^WW.1BL without secalin and with Glu-B3/Gli-B1 into elite wheat cultivars through marker-assisted backcross breeding

The wheat-rye 1RS.1BL translocation has been extensively utilized in wheat breeding programs across the world for multiple disease resistances, abiotic stress tolerance and higher grain yield. The Sec-1 locus present on the 1RS and codes for rye storage protein secalin which causes stickiness in the dough leading to poor bread quality. In addition to this, the absence of Glu-B3/Gli-B1 on 1BS in this translocation further deteriorates the bread-making quality of wheat. In the present study, Pavon MA1 was used as the donor parent, and two elite wheat cultivars HS240 and UP2338, having 1RS.1BL translocation, were used as the recipient parents. Pavon MA1 has 1RS(WW).1BL translocation in which the Sec-1 locus is absent whereas Glu-B3/Gli-B1 is present. This 1RS(WW).1BL translocation was introgressed in the two wheat cultivars using marker-assisted backcross breeding, and BC2F5 derivatives were developed for both the cultivars. The dough quality of these derivatives was analyzed through the micro-SDS-sedimentation test (MST), high-molecular-weight glutenin subunits (HMWGS), loaf volume and micro-dough laboratory test. The dough analysis showed that the elimination of secalin in the derivatives led to the higher loaf volume, dough development time, dough stability and water absorption thus improving its bread-making quality.