Measurement of the amount of chromosome aberration through object detection

Nature of the radiation which results in DNA damage is utilized to measure the extent of irradiation by quantification of the chromosome aberration. In this research, we applied You Only Look Once (YOLO), to measure the amount of the chromosome aberration in the chromosome slide automatically, based on the consideration that it requires both of identification and classification of the chromosome aberrations. Among the various models derived from YOLO, we selected YOLOv5. The parameters were updated by the objective function which is calculated as a summation of the complete intersection over union (CIoU) and classification loss. It is trained with the dataset, generated from attaching the single dicentric and normal chromosome images to the chromosome slide which contains only normal chromosome. The proposed model is shown to be localize and classify the chromosomes successfully. In conclusion, we confirmed the applicability of the object detection method for the purpose of measuring the amount of chromosome aberration.