Spatial continuous-flow polymerase chain reaction structure controlled by single-temperature driver

A spatial continuous-flow polymerase chain reaction microfluidic chip was designed with the traditional plane channel replaced by a verticalcavity channel by a single-temperature driver. To optimize the design, the temperature distribution in the microchannel of this chip was simulated by the finite-element method with various structural parameters and fluid velocities. The results show that the fluid produces three temperature zones of 94-95, 54-56, and 71-73 degrees C when the chip size is 54 mm (x) x 78 mm (y) x 47 mm (z), the wall thickness is 2 mm (x) x 20 mm (y) x 2 mm (z), and the pipe diameter is 1 mm. To increase the polymerase chain reaction amplification efficiency, we considered fluid velocities in the range of 0.0005-0.002 m/s. The optimal temperature for polymerase chain reaction amplification occurs at a fluid velocity of 0.0005 m/s. (c) 2023 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license http://creativecommons.org/licenses/by/4.0/).