The Mesoporous Polydopamine-Based Bowl-like Lipase Nanoreactor for the Biocatalytic Synthesis of Oleate Sterol Ester

Enzymatic esterification of phytosterols is essentialfor theirstability and bioavailability in lipophilic foods. In this study,we created a new polydopamine-based bowl-like mesoporous carrier (BLM-PDA)for lipase loading and catalyzed the esterification of phytosterolsand oleic acid to produce oleate sterol ester. Candida rugosa lipase (CRL) was selected to immobilize the polymer using physicaladsorption. SEM, TEM, XRD, TGA, XPS, FT-IR, and BET were used to characterizeit in order to confirm the successful preparation. These less-studiedasymmetric bowl-like particles showed much enhanced performance inall aspects. According to the findings, BLM-PDA had a lipase loadingof around 126.05 mg g(-1), and the thermal stabilityand reusability of BLM-PDA@CRL fared particularly well in activitytests. After 55 h, the catalyst successfully completed the oleatesterol ester yield of 81.07% of the transesterification cycle at 40 & DEG;C. Therefore, it offers more suggestions for using an immobilizedlipase nanoreactor to the biosynthesis of sterol esters.