New protocol to affix sample for environmental and high vacuum electron microscopy

Electron microscopy has become increasingly essential as an investigative research resource in various fields of science. Despite the evolution of the equipment and preparation techniques, little has been published on techniques for affixation and removal of samples on the stubs after analysis. This work aims to illustrate a quick and low-cost procedure for fixing and removing a sample from the support for analysis by electronic microscopy, applied to crustacean and insect species, as well as other invertebrates. It thus makes it feasible to use specimens or anatomical portions of specimens represented by a few individuals, or designated as paratypes or holotypes in a collection.Research HighlightsThis study presents a quick and low-cost procedure for fixing and removing samples from support for electron microscopy analysis.This technique makes it possible to use individual specimens or anatomical portions represented in a collection.Fluidization of the adhesive and its complete removal allow easy recovery and repositioning of samples. High-quality image of glossae of Ulmeritoides uruguayensis (Ephemeroptera, Insecta) produced using the new protocol for sample fixation on metal supports followed the new protocol.image