Mangiferin from Enicostemma littorale Blume with in silico and in vitro anti-inflammatory potential.

The bioassay-guided fractionation of the extract of aerial parts of resulted in two fractions 3 and 4 with moderate and potent antioxidant activity, respectively. The purification of fraction 3 gave swertiamarin (), while the LCMS profile of fraction 4 unveiled the presence of another constituent along with swertiamarin. The extensive purification of fraction 4 led to the unusual isolation of mangiferin () from . The uncommon isolation of mangiferin from motivated us to conduct its and screening as an anti-inflammatory agent. Both studies have proved mangiferin to be a promising anti-inflammatory molecule with a binding energy of -9.17 kcal/mol against Cyclooxygenase-2 protein and IC of 146.07 nanomolar. This study is the first report of the isolation of mangiferin, a xanthone glycoside from e.Communicated by Ramaswamy H. Sarma.