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堆肥用低温菌的筛选与鉴定

ZHANG Ting, FEN Man, ZHANG Yingying, ZHANG Ling,LI Xinchang,LI Haiyan,SHI Aili, LI Jie

Anhui Agricultural Science Bulletin(2023)

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Abstract
为解决我国北方地区秋冬季节温度低,农业废弃物发酵腐熟慢、周期长等问题,本研究以冷凉地区农业废弃物为材料,采用平板涂布法分离筛选低温菌群,并利用选择培养基对菌株进行功能复筛,以期为冷凉地区农业废弃物低温快速发酵提供菌种资源.结果表明,共分离出低温菌9株,功能复筛确定其中4株菌株具有固氮、溶磷及分解酪蛋白等功能,经鉴定这4株功能菌分别为Pantoea coffeiphila、米氏假单胞菌(Pseudomonas migu-lae)、Pseudomonas canadensis和盖氏假单胞菌(Pseudomonas gessardii).其中,Pantoea coffeiphila具有固氮和溶解有机磷功能;Pseudomonas migulae和Pseudomonas canadensis能够溶解有机磷、无机磷,并且具有分解酪蛋白的功能;Pseudomonas gessardii能够分解酪蛋白.
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Key words
low temperature bacteria,screening and identification,functional bacteria,ferment
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