Enhanced oxidase-mimic constructed by luminescent carbon dots loaded on MIL-53(Fe)-NO2 for dual-mode detection of gallic acid and biothiols in food and humans
FOOD CHEMISTRY(2024)
摘要
Monitoring of gallic acid (GA) in food and biothiols in humans is crucial for body health. Nanozyme-mediated colorimetric strategy for evaluating them has been widely applied nowadays, however, the inferior efficient and susceptible single-signal recognition limit its further application. Herein, a sensitive biosensor was first constructed for bimodal detection of GA and biothiols based on CDs@MIL-53(Fe)-NO2, prepared through a facile and time-saving microwave treatment. Benefiting from the excellent fluorescent and electron transfer properties of CDs, CDs@MIL-53(Fe)-NO2 exhibited significant enhanced blue fluorescence and oxidase-like activity, which could oxide colorless 3,3 & PRIME;,5,5 & PRIME;-tetramethylbenzidine without H2O2, and the blue product could quench the fluorescence of composite. The dual-mode assay based on such bifunctional nanozyme showed an extremely sensitivity towards GA/L-cysteine/homocysteine with the detection limit of 62/65/124 nM and 17/16/27 nM in colorimetric/fluorescent modes, respectively. The practicability in real samples and portability based on a smartphone of the analysis has been investigated with reliable results.
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关键词
Oxidase-mimic,Carbon dots,Metal-organic framework,Fluorescence,Colorimetry,Gallic acid
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