玉米褪绿斑驳病毒分子检测方法研究进展

LI Xian-Feng, YU Xuan,HU Shu-Qing, LI Pan-Pan,FENG Li-Xia, YUAN Jun-Jie,SHAO Bao-Lin, WEI Shuang

China Port Science and Technology(2023)

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Abstract
玉米褪绿斑驳病毒(maize chlorotic mottle virus,MCMV)是全球玉米种植产业最重要的病害之一,其快速、精准检测和鉴定方法在检疫防控中至关重要.本文综述了现有的玉米褪绿斑驳病毒分子检测方法的研究进展,总结了基于变温扩增的RT-PCR、RT-qPCR和多重RT-PCR方法,基于等温扩增的RT-LAMP、RT-RPA和RT-RAA方法,以及CRISPR/Cas系统和高通量测序等检测方法的优缺点,可根据不同情况选择合适的方法进行玉米褪绿斑驳病毒检测.最后,展望了新型检测方法在玉米褪绿斑驳病毒检测中的应用和发展趋势.
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Key words
maize chlorotic mottle virus (MCMV),molecular detection,research progress
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