Decreased Expression of CD314 by NK Cells Correlates with Their Ability to Respond by Producing IFN-? after BCG Moscow Vaccination and Is Associated with Distinct Early Immune Responses

The immune response to vaccines is complex and results in various outcomes. BCG vaccination induces innate and specific responses that can lead to protection against tuberculosis, and cross-protection against other infections. NK cells have been associated with BCG-induced protection. Therefore, we hypothesize that differences in NK cell status before BCG vaccination may have a role in the ability of BCG to activate the immune response. Participants of a clinical trial were evaluated after BCG vaccination. The participants were assigned to different groups according to variation in IFN-? expression by NK cells between days 1 and 15 after BCG vaccination. Individuals that presented a higher increase in IFN-? expression by NK cells presented reduced CD314 expression at day 1, and after vaccination an increase in inflammatory NK cells and CD4 T-cell expression of IL-17. A negative correlation between expression of CD314 at day 1 and that of IFN-? by NK cells after BCG vaccination was observed. Participants with lower of IFN-? expression by NK cells after BCG vaccination presented an increase in the cytotoxic NK subpopulation and CD4 T-cell expression of IL-17 and IFN-?. In conclusion, the expression of CD314 by NK cells before BCG vaccination influences their IFN-? responses, generation of NK subpopulations, and the specific T immune response at 15 days after vaccination.