Separation of epithelial and immune cells from biopsy samples.
Methods in cell biology(2023)
Abstract
Celiac disease (CD) is a chronic and autoimmune disease that develops in genetically predisposed individuals upon exposure to dietary gluten. The availability of the target tissue for research has made it possible to identify alterations in the transcriptome and methylome in the celiac gut. However, gene expression and methylation is highly variable among different cell types, and separation of cellular populations in target tissue must be considered for the understanding of the specific cellular and immune responses to gluten. In this context, a few studies have demonstrated that focusing on an isolated cell population, novel candidate genes involved in the pathogenesis of the disease can be identified. Here, we describe a method to separate epithelial and immune cells from biopsy samples for DNA and RNA isolation. With minor variations, the same technique can be applied to other tissues and cell types.
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Key words
CD45 antigen,Cell adhesion molecule EpCAM (CD326),Duodenal biopsy,Enterocytes,Epithelial cells,Immune cells,Isolated cell population,Leukocytes,Separation of cell populations
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