The inhibitory effects of N-myc down-stream regulated gene-2 on proliferation of bladder carcinoma cell line T24 in vitro

Objective To detect the expression level of N-MYC dwn-stream rgulated gne-2(NDRG2) in different prostatic carcinoma cell lines, and investigate the inhibitory effects of NDRG2 on proliferation of PC3 cell line mediated by adenovirus in vitro. Methods The expression of NDRG2 mRNA and protein was detected by using RT-PCR and Western blot respectively, pAD_CMV_NDRG2 adenoviru-ses were used to infect the PC3 cells. The effects of the adenovirus on cell proliferation were observed by u-sing MTr method. The changes in cell cycle and apoptosis were detected by flow cytometry at the 48th and 72nd h after the infection. Results The results of RT-PCR and Western blot indicated that the three pros-tatic carcinoma cell lines had relatively lower NDRG2 mRNA and protein levels. PC3 cells had the lowest level among the three cell lines. MTr method showed that NDRG2 began to suppress the proliferation of the PC3 cells at the 36th h (F≥ 12.43 ,P ＜0.01). Compared with the blank control and the positive control, pAD_CMV_NDRG2-infected cells showed an increase in G1 phase at the 72nd h (68.06% ,50.33% and 50.33% respectively). Furthermore,NDRG2-induced apoptosis was also increased at the 72nd h in com-parison with the controls (12.33% ,3.21% and 3.87% respectively). Conclusion NDRG2 might be in-volve in the pathogenesis of the prostatic carcinoma. Adenovirus-mediated NDRG2 could suppress the pro-liferation of the PC3 cells significantly. Key words: Prostatic carcinoma; Protooneogene; Adenovirus