Mp14-04 establishment of blood-based diagnostic markers of urothelial carcinoma using chiral amino acids

You have accessJournal of UrologyCME1 Apr 2023MP14-04 ESTABLISHMENT OF BLOOD-BASED DIAGNOSTIC MARKERS OF UROTHELIAL CARCINOMA USING CHIRAL AMINO ACIDS Atsunari Kawashima, Akinaru Yamamoto, Sassi Nesrine, Toshihiro Uemura, Tomonori Kimura, Masashi Mita, Toshiki Oka, Yohei Okuda, Gaku Yamamichi, Eisuke Tomiyama, Yu Ishizuya, Yoshiyuki Yamamoto, Taigo Kato, Koji Hatano, Ryoichi Imamura, and Norio Nonomura Atsunari KawashimaAtsunari Kawashima More articles by this author , Akinaru YamamotoAkinaru Yamamoto More articles by this author , Sassi NesrineSassi Nesrine More articles by this author , Toshihiro UemuraToshihiro Uemura More articles by this author , Tomonori KimuraTomonori Kimura More articles by this author , Masashi MitaMasashi Mita More articles by this author , Toshiki OkaToshiki Oka More articles by this author , Yohei OkudaYohei Okuda More articles by this author , Gaku YamamichiGaku Yamamichi More articles by this author , Eisuke TomiyamaEisuke Tomiyama More articles by this author , Yu IshizuyaYu Ishizuya More articles by this author , Yoshiyuki YamamotoYoshiyuki Yamamoto More articles by this author , Taigo KatoTaigo Kato More articles by this author , Koji HatanoKoji Hatano More articles by this author , Ryoichi ImamuraRyoichi Imamura More articles by this author , and Norio NonomuraNorio Nonomura More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000003234.04AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: There are no clinically established blood-based diagnostic biomarkers for urothelial carcinoma patients. Amino acids (AA) have two isoforms of L- and D-AA and D-AA have been believed to be physiologically nonexistent. However, it becomes clear that some D-AA has clinical significance in the human body. Here, we evaluated circulating D-AA levels in the blood and their clinical possibilities as diagnostic biomarkers in urothelial cancer patients. METHODS: We enrolled 345 patients just before surgery for urothelial carcinoma (UC) (n=123), renal cell carcinoma (RCC) (n=143), and healthy donor of kidney transplantation (n=79). We collected 345 serum samples just before surgery and 16 samples one week after surgery. D-AA levels in serum were measured using two-dimensional high-performance liquid chromatography. We evaluated its usefulness as a diagnostic biomarker of UC in the derivation cohort (n=22) and two independent validation cohorts in 254 (Cohort-1) and 69 (Cohort-2) participants, respectively. RESULTS: In the derivation cohort, only four D-AA (D-Asparagine (D-Asn), D-Serine (D-Ser), D-Alanine (D-Ala), and D-Proline (D-Pro)) could be measured in serum samples among 22 kinds of D-AA. D-Asn and D-Ala levels were significantly higher in UC patients compared to RCC patients and healthy donors (p<0.05 and p<0.01, respectively). The area under the curve (AUC) of each D-Asn to diagnose UC was 0.800. Sensitivity and specificity were 100% and 60% using a cut-off level of 0.233 IU/ml. In validation Cohort-1, D-Asn levels had a high accuracy of diagnosis for UC (AUC: 0.851). Sensitivity and specificity were 77.8% and 79.3% using the same cut-off level as the derivation cohort. In validation cohort-2, D-Asn levels had the high accuracy of diagnosis for UC (AUC: 0.836) and its sensitivity and specificity were 61.9% and 81.2% using the same cut-off level as the derivation cohort. Also, serum D-Asn levels of 16 UC patients just after surgery significantly decreased compared to those just before surgery (p=0.005). CONCLUSIONS: D-Asn levels in the blood could become the promising diagnostic markers of UC patients. Source of Funding: This work was supported by a KAKENHI grant (22H03213) © 2023 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 209Issue Supplement 4April 2023Page: e183 Advertisement Copyright & Permissions© 2023 by American Urological Association Education and Research, Inc.MetricsAuthor Information Atsunari Kawashima More articles by this author Akinaru Yamamoto More articles by this author Sassi Nesrine More articles by this author Toshihiro Uemura More articles by this author Tomonori Kimura More articles by this author Masashi Mita More articles by this author Toshiki Oka More articles by this author Yohei Okuda More articles by this author Gaku Yamamichi More articles by this author Eisuke Tomiyama More articles by this author Yu Ishizuya More articles by this author Yoshiyuki Yamamoto More articles by this author Taigo Kato More articles by this author Koji Hatano More articles by this author Ryoichi Imamura More articles by this author Norio Nonomura More articles by this author Expand All Advertisement PDF downloadLoading ...