Palladium-based mass tag cell barcoding with a doublet-filtering scheme and single-cell deconvolution algorithm

Eli R Zunder,Rachel Finck,Gregory K Behbehani,El-ad D Amir,Smita Krishnaswamy,Veronica D Gonzalez,Cynthia G Lorang,Zach Bjornson,Matthew H Spitzer,Bernd Bodenmiller,Wendy J Fantl,Dana Pe'er,Garry P Nolan

Nature Protocols（2015）

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摘要

This protocol describes how to use mass-tag cell barcoding to label individual cell samples with unique combinatorial barcodes. The samples can then be pooled for processing and measurement on a mass cytometer.

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关键词

mass tag cell,single-cell cell,palladium-based,doublet-filtering

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