Probing the interaction of iminium form of sanguinarine with human salivary α-amylase by multi-spectroscopic techniques and molecular docking

•HSAmy non catalytic interaction with sanguinarine (Iminium) was explored.•The major interaction was hydrophobic bond.•The interactions perturbed HSAmy structures and surface hydrophobicity.•The ligand preferentially bound to the HSAmy active site.•HSAmy is a reluctant surrogate payload for sanguinarine (Iminium)